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E    spheroid@happy-cell.com

Any Questions?

In-order to ensure that our application scientists can provide you with the correct information please answer the following questions.

Email Address:
What type of culture vessel are you using?: (e.g. culture flask, 96 well plate etc)
What type of cells are you using?: 
What Volume are you maintaining the cells in?:
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Happy Cell Dilution Scheme

Product Information

Optimised suspension media that enables the 3D culture of cells. Uniquely formulated, low viscosity solution for High Throughput Screening (HTS) and High Content Screening (HCS) cell based assays and High Throughput Flow Cytometry… Pre formulated with RPMI, DMEM or whatever your preferred culture medium is.


  • Flow Cytometry
  • Toxicology studies
  • Suspension medium for spheroid growth
  • Stem Cells & Primary Cells
  • Bioreactor growth medium for non-adherent cells e.g. T Lymphocytes

Instructions for use

Store at 2-8°C until ready to use.

  • Operating range 10-37°C
  • Wipe down outside of container with 70% ethanol before opening
  • Contains 50 I.U/ml of Penicillin & Streptomycin
  • Ready for use, simply add FCS and other nutritional requirements
  • Do not freeze
Happy Cell Benefits

Spheroid optimisation protocol

Step 1 Preparing cells for seeding

If an enzymic dissociation step (such as trypsinisation) is required ensure that cells are washed twice in fresh media as per your normal protocol. Spin cells down into a pellet and carefully remove all/or as much supernatant as possible.  Re-suspend cells in pre-warmed 1x happy cell media at a density of 100,000 cells per ml.

Step 2 Cell Seeding

Titrate cells  at densities ranging from 10,000 to 1000 cells /100ul/well into low binding clear bottom 96-well plate and incubate for a minimum of 72 hours at 37°C/ 5% CO2 and 95% humidity. Check plates using microscope with low power objective at least once daily to assess optimal seeding density and incubation times.

Advanced Microplate US Patent Granted!!!


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